- Alkaline phosphatase enzyme detection system
- Avidin/biotin technology
- High sensitivity/low background
- Low cost
- Preferred for tissues that have high endogenous peroxidase activity
- Offers additional substrate choices
- Used as a second label in multiple antigen labeling applications
- Available without (Standard kit) or with biotinylated species-specific antibody
- 2 ml Reagent A (Avidin DH) plus 2 ml Reagent B (Biotinylated AP)
With more than 50,000 citations to its credit, the VECTASTAIN[R] ABC kit remains widely popular. Based on the versatile avidin-biotin complex interaction, the system is modular, and along with our selection of secondary antibodies, can accommodate a wide array of primary antibody and tissue species. Our ABC kits are economical and continue to be a staple product in any immunohistochemistry (IHC) and immunocytochemistry (ICC) laboratory.
The sensitivity of the VECTASTAIN[R] ABC-AP system is comparable to that of the peroxidase VECTASTAIN[R] Elite [R]ABC system. The VECTASTAIN[R] ABC-AP Kits may be preferred for tissues that have high endogenous peroxidase activity. The system also offers additional substrate color choices.
Reagent A and B when mixed form the ABC-AP complex.Kit is sufficient to stain approximately 1000-2000 sections or fifty 100 cm2 blots.
The VECTASTAIN[R] ABC systems are extremely sensitive due to the form and number of active enzyme molecules associated with the preformed Avidin/Biotinylated enzyme Complex. This ABC complex takes advantage of two important properties of avidin: 1) an extraordinarily high affinity for biotin (over one million times higher than antibody for most antigens), and 2) four biotin-binding sites. These properties allow macromolecular complexes (ABCs) to be formed by mixing Avidin DH (Reagent A) with its paired biotinylated enzyme (Reagent B) prior to use. The ABC reagent, once formed, remains stable for many hours after formation and can be used for several days after preparation.
The VECTASTAIN[R] ABC Reagent can be used to detect any molecule that is biotinylated. This property gives the avidin-biotin complex (ABC) method great versatility in the types of targets that can be detected as well as the types of applications in which it can be employed. Biotinylated primary antibodies, secondaries, lectins, neuronal tracers, nucleic acids, and ligands can be effectively visualized in applications such as:
All applications benefit from the high sensitivity, low background, reproducibility, and economy of the VECTASTAIN[R] ABC system.