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Antibodies

VAChT, Polyclonal Antibody

Product Sizes
50 ug
139-103-SY-50UG
Academic Pricing
Please contact us for academic pricing.
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About this Product
SKU:
139-103-SY
Additional Names:
SLC18A3, vesicular Neurotransmitter transporter, Acetylcholine transporter, spinal cord|VAChT
Application:
Conjugation/Labeling, IHC-Paraffin, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Buffer:
PBS
Clonality:
Polyclonal
Extra Details:
50 ug specific antibody, lyophilized. Affinity purified with the immunogen. Albumin and azide were added for stabilization. For reconstitution add 50 ul H2O to get a 1mg/ml solution in PBS. Then aliquot and store at -20°C until use.
Gene Details:
Slc18a3
Host:
Rabbit
Immunogen:
Recombinant protein corresponding to AA 475 to 530 from rat VAChT (UniProt Id: Q62666)Recombinant protein corresponding to residues near the carboxy terminus of rat VAChT
Molecular Weight:
56.55
Physical State:
Lyophilized
Protein Details:
Q62666
Purification:
Affinity Purified
Reactivities:
Fish, Human, Mouse, Porcine, Rat
Shipping Conditions:
Blue Ice
Specificity:
VAChT
Storage Conditions:
2-8[o]C
Size:
50 ug
Supplier:
Synaptic Systems
Type:
Antibody: Polyclonal Antibody
Synonyms:
rVAT;solute carrier family 18 (vesicular acetylcholine transporter), member 3;solute carrier family 18 (vesicular acetylcholine), member 3;solute carrier family 18 (vesicular monoamine) member 3;Solute carrier family 18 member 3;solute carrier family 18, member 3;VACht;vesicular acetylcholine transporter
  • Manufacturer's Data Sheet:139103
Antibody dilution: 1 : 1000 Sample: enriched synaptic vesicles from rat brain (LP2) Detection: AP-staining
Indirect immunostaining of PFA fixed rat neurons with rabbit anti-VAChT (cat. no. 139 103, dilution 1 : 500; red) and mouse anti-Synapsin 1 (cat. no. 106 001, dilution 1 : 1000; green). Nuclei have been visualized by DAPI staining (blue).
Indirect immunostaining of cholinergic markers in the rat basal forebrain with anti-VAChT ( dilution 1 : 100; red) and a commercially available goat anti-ChAT antibody (dilution 1 : 25, green). Immunoreactive structures were revealed with Cy3 and Cy5 conjugated secondary antibodies. Courtesy: W. Hartig, Paul Flechsig Institute, Leipzig
Indirect immunostaining of PFA fixed mouse spinal cord section with rabbit anti-VaChT (cat. no. 139 103, dilution 1 : 500, red). Nuclei have been visualized by DAPI staining (blue).
Indirect immunostaining of PFA fixed mouse heart section with rabbit anti-VaChT (cat. no. 139 103, dilution 1 : 500, red). Nuclei have been visualized by DAPI staining (blue).