FluoroSpot Plus: Human IFN-G Gamma/IL-4/IL-5
Product Sizes
2 plates
FSP-011608-2-2PLATES
About this Product
- SKU:
- FSP-011608-2
- Application:
- FLUOROSPOT
- Extra Details:
- The FluoroSpot assay is an inventive development of the ELISpot assay and uses fluorescent detection instead of colorimetric. This allows for simultaneous detection of several analytes, which is a major advantage. The human IFN-G Gamma/IL-4/IL-5 FluoroSpot kit is perfect for users who wish to have a convenient assay with minimized assay variability. It enables enumeration of cells secreting one, two or three analytes. The kit includes low fluorescent plates pre-coated with monoclonal capture antibodies, monoclonal detection antibodies, secondary reagents conjugated to fluorophores, a fluorescence enhancer and an anti-CD28 monoclonal antibody for co-stimulation. A polyclonal activator for human T cells (mAb CD3-2) is supplied as a positive control for cytokine secretion. For analysis, we recommend the use of an automated FluoroSpot reader equipped with filters for excitation 490 nm/emission 510 nm (FITC) for 490-reagent detection, excitation 550 nm/emission 570 nm (Cy3) for 550-reagent detection and excitation 640 nm/ emission 660 nm (Cy5) for 640-reagent detection. Filters should have high specificity to avoid bleed-through artifacts.|Ensure reproducibility when diving into a multiplex analysis.|This FluoroSpot Plus kit is intended for the enumeration of cells secreting human IFN-G Gamma, IL-4, and IL-5 using the FluoroSpot assay. For research use only. Not for use in diagnostic procedures.
- Format:
- Plus, 3-analyte fluorescence
- Immunogen:
- IFN-γ,IL-4,IL-5
- Reactivities:
- Human
- Shipping Conditions:
- Blue Ice
- Specificity:
- This kit is based on matched pairs of mAbs specific for human IFN-G Gamma, IL-4, and IL-5.
- Storage Conditions:
- Please refer to datasheet
- Supplier:
- Mabtech
- Type:
- Assay Kits: Fluorospots
