SUMO3-CHOP2 Reporter DeSUMOylation Assay
Product Sizes
30 reactions
PR-1102-0375-30REACTIONS
About this Product
- SKU:
- PR-1102-0375
- Extra Details:
- The SUMO3-CHOP2-Reporter DeSUMOylation Assay consists of human SUMO3 fused to a reporter enzyme, as well as a separate substrate for the reporter enzyme. When fused to SUMO3, the reporter is rendered catalytically inactive. Following cleavage of the SUMO3-reporter system by the isopeptidase, free (and now active) reporter subsequently acts upon its substrate. Thus, in this coupled assay, the signal generated by cleavage of the reporter enzyme's substrate is a quantitative measure of isopeptidase activity. \nContents \n-- SUMO3-CHOP2-Reporter System: 1 x 375 uL (4 uM) \n-- SENP2 core (Control Isopeptidase): 2 x 10 uL (2 uM) \n-- Reporter Substrate 2: 1 x 30 uL (25 uM) \n-- Control Fluorophore 2 :1 x 20 uL (1 mM)
- Shipping Conditions:
- Dry Ice
- Storage Conditions:
- -70[o]C
- Supplier:
- LifeSensors
- Type:
- Assay Kits: Detection