HighYield T7 AF647 RNA Labeling Kit (UTP-based), Preparation of randomly AF647-modified RNA probes by in vitro transcription with UTP-PEG5-AF647
Product Sizes
20 reactions
RNT-101-AF647-20REACTIONS
About this Product
- SKU:
- RNT-101-AF647
- Conjugate:
- Alexa Fluor® 647
- Extra Details:
- HighYield T7 AF647 RNA Labeling Kit is designed to produce randomly AF647-modified RNA probes via in vitro transcription. Such probes are ideally suited for in situ hybridization and Northern Blot experiments. AF647 (a structural analog to Alexa Fluor® 647) is a hydrophilic dye with increased photostability compared to Cy5. UTP-PEG5-AF647 is efficiently incorporated into RNA as substitute for its natural counterpart UTP using an optimized reaction buffer and T7 RNA Labeling Polymerase Mix. 35 % UTP-PEG5-AF647 substitution typically results in an optimal balance between reaction and labeling efficiency. Individual optimization of UTP-PEG5-AF647/UTP ratio however, can easily be achieved with the single nucleotide format. The resulting AF647-modified RNA probe can subsequently be detected by fluorescence spectroscopy. The kit contains sufficient reagents for 20 labeling reactions of 20 ul each (35 % UTP-PEG5-AF647 substitution, 2.5 mM ATP, GTP, CTP, 0.2 mM UTP, 0.1 mM UTP-PEG5-AF647). A 20 ul reaction yields about 4-6 ug RNA after 30 min incubation (1 ug T7 control template, 1.4 kb RNA transcript). Yields may however vary depending on the template (promotor design, sequence length, secondary structure formation).
- Shipping Conditions:
- Blue Ice
- Storage Conditions:
- -20[o]C Aliquot. Avoid freeze/thaw cycles.
- Supplier:
- Jena Bioscience
- Type:
- Assay Kits: Labelling