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Antibodies

Anti-Phosphotyrosine Antibody

Product Sizes
100 ul
PM3751-100UL
About this Product
SKU:
PM3751
Additional Names:
Phosphotyrosine mAb
Application:
Immunocytochemistry, Immunoprecipitation, Western Blot
Buffer:
PBS
CE/IVD:
RUO
translate.label.attr.clone:
M375
Clonality:
Monoclonal
Conjugate:
p-Tyr - Phosphorylated
Extra Details:
Our Anti-Phosphotyrosine phosphospecific primary antibody from PhosphoSolutions is mouse monoclonal. It detects chicken, human, mouse, and rat Anti-Phosphotyrosine and is Protein A purified. It is great for use in WB, ICC, IP.
Format:
Protein A Purified
Gene Details:
N/A
Host:
Mouse
Immunogen:
Clone M375 was generated from tyrosine phosphorylated proteins purified from pervanadate treated A431 cells.
Isotype:
IgG2b
Molecular Weight:
N/A
Physical State:
Liquid
Reactivities:
Avian, Human, Mouse, Rat
Shipping Conditions:
Blue Ice
Specificity:
This antibody detects phosphotyrosine-containing proteins on SDS-PAGE immunoblots of EGF treated A431 cells, as well as pervanadate treated A431, HeLa, Jurkat, and endothelial cells.
Storage Conditions:
-20[o]C Avoid freeze/thaw cycles.
Type:
Antibody: Monoclonal Antibody
Immunocytochemical labeling of phosphotyrosine in control and pervanadate-treated A431 cells. The cells were labeled with rabbit polyclonal anti-Phosphotyrosine (PP2221) and mouse monoclonal anti-Phosphotyrosine (PM3751), then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.
Western blot of HeLa cells treated with pervanadate (1 mM) for 30 min. Phosphotyrosine containing proteins were immunoprecipitated with rabbit polyclonal anti-Phosphotyrosine:Agarose (Lane 1) or with no antibody agarose beads (Lane 2), and blots were made that included the whole lysate (Lane 3). The blots were probed with mouse monoclonal anti-Phosphotyrosine (PM3751) to detect phosphotyrosine containing proteins.