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Assay Kits & ELISAs

Dual Caspase 1 and Mitochondrial Membrane Potential Assay Kit

Product Sizes
100 tests
MITCAP600-2-100TESTS
About this Product
SKU:
MITCAP600-2
Additional Names:
Cell Viability Assay
Application:
ELISA
Extra Details:
This kit utilizes a cell permeable two-color stain to simultaneously detect caspase activity and mitochondrial membrane potential (MMP) in cells. Active caspase-1 is detected with the caspase probe FAM-YVAD-FMK and MMP with a cationic dye.
Immunogen:
Caspase-1
Shipping Conditions:
Blue Ice
Storage Conditions:
2-8[o]C
Type:
Assay Kits: Cell Viability
Figure 1. Jurkat cells were stimulated with staurosporine (B) or DMSO (A), then stained according to the kit protocol. The cells were washed twice and analyzed by flow cytometry: Ex:488nm Em: FL1 and FL2. A. Healthy cells show a strong red fluorescence (intact mitochondria) and no green fluorescence (no active caspases). B. Apoptotic cells show a loss of red fluorescence (y axis) due to loss of mitochondrial membrane potential and positive green fluorescence (x axis) indicating active caspases.
Figure 2. HeLa Cells activated with staurosporine (B) or DMSO (A), then stained according to the kit protocol. The cells were washed twice and analyzed by flow cytometry: Ex:488nm Em: FL1 and FL2. A. Healthy cells show a strong red fluorescence (intact mitochondria) and no green fluorescence (no active caspases). B. Cells becoming apoptotic show loss of red fluorescence (y axis) due to loss of mitochondrial membrane potential and positive green fluorescence (x axis) indicating active caspases.
Figure 3. Flow cytometer set up: set flow cytometer so that cells can be gated with a forward scatter vs side scatter plot.
Figure 4. Flow cytometer set up: set up dot plot histogram of FL2 vs FL1.