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Antibodies

anti-CD274 / PD-L1 antibody [SQab1716]

Product Sizes
50 ul
ARG65862-50UL
100 ul
ARG65862-100UL
Academic Pricing
Please contact us for academic pricing.
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About this Product
SKU:
ARG65862
Additional Names:
Programmed cell death 1 ligand 1; B7-H1; B7H1; PDL1; PDCD1 ligand 1; B7 homolog 1; PD-L1; CD antigen CD274; PDCD1L1; B7-H; Programmed death ligand 1; PDCD1LG1
Application:
Flow Cytometry, IHC-Paraffin, Immunocytochemistry, Immunofluorescence, Immunoprecipitation
Buffer:
PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.
CE/IVD:
RUO
translate.label.attr.clone:
SQab1716
Clonality:
Monoclonal
Extra Details:
CD274 / PD-L1 is an immune inhibitory receptor ligand. It is expressed by hematopoietic and non-hematopoietic cells, such as T cells and B cells and various types of tumor cells. The encoded protein is a type I transmembrane protein that has immunoglobulin V-like and C-like domains. Interaction of this ligand with its receptor inhibits T-cell activation and cytokine production. During infection or inflammation of normal tissue, this interaction is important for preventing autoimmunity by maintaining homeostasis of the immune response. In tumor microenvironments, this interaction provides an immune escape for tumor cells through cytotoxic T-cell inactivation. Expression of this gene in tumor cells is considered to be prognostic in many types of human malignancies, including colon cancer and renal cell carcinoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
Host:
Rabbit
Immunogen:
Recombinant protein around aa. 19-239 (extracellular) of Human CD274 / PD-L1.
Isotype:
IgG
Purification:
Protein A Purified; Affinity Purified
Reactivities:
Human
Shipping Conditions:
Blue Ice
Storage Conditions:
-20[o]C
Supplier:
Arigo Biolaboratories
Type:
Antibody: Monoclonal Antibody
Flow Cytometry: 293 cells transfected with PD-L1 gene and stained with ARG65862 anti-CD274 / PD-L1 antibody [SQab1716] at 1:200, in 1x PBS/1% BSA for 30 min at room temperature, followed by Alexa Fluor® 488 labelled secondary antibody (red histogram). Unlabelled sample (black histogram) was used as a control.
Immunofluorescence: 293 cells trasnfected with PD-L1 gene. Cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% goat serum for half an hour at room temperature. Samples were stained with ARG65862 anti-CD274 / PD-L1 antibody [SQab1716] (green) at 1:10000 at 4°C. DAPI (blue) was used as the nuclear counter stain.
Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded sections of Human hepatocellular carcinoma tissue stained with ARG65862 anti-CD274 / PD-L1 antibody [SQab1716] at 1:200 dilution. Antigen Retrieval: Boil tissue section in Tris/EDTA buffer (pH 9.0).
Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded sections of Human lung adenocarcinoma tissue stained with ARG65862 anti-CD274 / PD-L1 antibody [SQab1716] at 1:200 dilution. Antigen Retrieval: Boil tissue section in Tris/EDTA buffer (pH 9.0).
Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded sections of Human placenta tissue stained with ARG65862 anti-CD274 / PD-L1 antibody [SQab1716] at 1:200 dilution. Antigen Retrieval: Boil tissue section in Tris/EDTA buffer (pH 9.0).