Glysite Explorer

Many questions remain unanswered on the spatial biology of glycoproteins, despite changes in the glycocalyx being observed in a wide range of conditions from neurological degenerative diseases and cancers.

Analysis of glycoproteins has previously relied heavily on techniques that require glycosylated proteins to be extracted from cells and then further analysed with a series of tests and glycan digestions to reveal the glycobiology in detail. Where specifically glycosylated proteins have been located in cells has been hard to achieve, until now.

Vector Laboratories have released the Glysite Explorer in situ Proximity Ligation Assay Kit to enable researchers to pinpoint specifically glycosylated target proteins in their spatial biology assays. The kit uses a combination of a primary antibody and a lectin to produce a glycoprotein specific label.

Schematic of Glysite™ Explorer in situ PLA Glycan Detection Kit. A. Lectin binds to the target glycan and primary antibody binds to the target protein. B. Oligo probes bind to the primary antibody and lectin. C. When oligo probes are in proximity (~40 nm), ligation occurs. D. Amplification and detection produces a coloured precipitate at the site of proximity. Created with BioRender.com, curtesy of Vector Laboratories Inc.

Click here to find out more about how Glysite™ Explorer can help advance your research today!

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