SignalChem Diagnostics

SignalChem Diagnostics CRISPR-Cas9

2BScientific are proud to distribute SignalChem Diagnostics range of high-quality CRISPR reagents, ensuring reliability in a range research applications. CRISPR has revolutionised molecular biology, enabling precise gene editing, diagnostics, and therapy development. CRISPR associated proteins provide powerful tools for DNA and RNA manipulation.

CRISPR-Cas

Cas9

Precise DNA Editing via Cas9 is utilised for precise DNA targeting and cleavage. It recognises sequences via a simple 5’-NGG-3’ PAM, making it highly adaptable. SignalChem’s engineered variants allow researchers to customise DNA modifications, including double-strand cuts, strand-specific nicking, or target binding without cleavage. SignalChem additionally offer Glycerol-free formulations to ensure suitability for sensitive applications.

Cas12

Advanced DNA targeting and detection using Cas12 expands CRISPR’s potential with staggered cuts and collateral single-stranded DNA (ssDNA) cleavage, making it a powerful option for molecular diagnostics and research. SignalChem offers multiple Cas12 options that are optimised for different experimental conditions, with advantages like broader PAM recognition and low-temperature activity. Additionally, Cas12’s collateral cleavage enhances DNA detection with high specificity.

Csm6: Enhanced RNA Detection

Csm6 is an RNA-activated endoribonuclease involved in the CRISPR-Cas immune system, specifically in type III CRISPR complexes. It plays a crucial role in bacterial defense by degrading viral RNA, enhancing immunity against phage infections. In research, Csm6 is studied for its potential in programmable RNA degradation, pathogen detection, and synthetic biology applications.

SignalChem Diagnostics Product Range
Cas9	Cas13
Double Stranded Blunt Cuts	RNA Targeting
Strand Specific Nicking	Enhanced Thermostability
Target Binding without Cleavage	Structural and Conformational Differences
Cas12	Csm6
crRNA-Only System	Cyclic Hepta-adenylate Activating Ligand
Active at Lower Temperatures	Cyclic Tetra-adenylate Activating Ligand
Smaller Size (Requires crRNA and tracrRNA)

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Csm6: Enhanced RNA Detection