600-401-DU0
PST1 Antibody
Cannot supply to this region.
- SKU:
- 600-401-DU0
- Additional Names:
- PST1|Pst1 Antibody, HPF2, HPF2, YDR055W, D4214, YD9609.09, Cell wall mannoprotein PST1, Haze protective factor 2
- Application:
- ELISA, WB
- Concentration:
- 1 mg/ml
- Physical State:
- Liquid
- Species Reactivity:
- Human
- Storage Conditions:
- -20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles., 2-8[o]C diluted. Aliquot. Avoid freeze/thaw cycles.
- Supplier:
- Rockland Inc
- Host:
- Rabbit
- Reactivities:
- Yeast
- Buffer:
- 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride
- Immunogen:
- Anti-Pst1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide from the N-terminus of yeast Pst1.
- Formulation:
- 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
- Uniprot:
- Q12355
- Synonyms:
- Haze protective factor 2;HPF2;Protoplast secreted protein 1;Pst1p;YDR055W
- Extra Details:
- Protoplast secreted protein 1 (Pst1) is an anchored plasma membrane protein. Pst1 was previously identified as a protein secreted by yeast regenerating protoplasts, which suggests a role in cell wall construction. Pst1 is a protein with 444 amino acids and is attached to yeast cell wall via a glycosylphosphatidylinisotol (GPI) anchor. Pst1 contains 15 potential N-linked glycosylation sites and is heavily glycosylated. It migrates at approximately 200 kDa on SDS-PAGE when produced in wild type S. cerevisiae. Our Pst1 protein (Catalog No. 95-151) was expressed in a genetically manipulated triple-mutant (TM) S. cerevisiae stain (D Deltaoch1 D Deltamnn1 D Deltamnn4), which results in the production of sole Man8GlcNAc2 carbohydrate structures and Pst1 migration at approximately 100 kDa. When produced in the TM yeast, Pst1 can be recognized by several glycan-specific HIV-1 broadly neutralizing antibodies, including 2G12 and recently identified PGT antibodies. Among several heavily N-glycosylated TM yeast glycoproteins, Pst1 shows higher affinity for 2G12 and efficiently inhibits gp120 interactions with 2G12 and DC-SIGN, and it also blocks 2G12-mediated neutralization of HIV-1 pseudoviruses.
- Shipping Conditions:
- Dry Ice