200-401-MU6
Nuclease/NucA Antibody
Cannot supply to this region.
- SKU:
- 200-401-MU6
- Additional Names:
- Benzonase, Nuclease, Endonuclease, Serratia marcescens endonuclease, NucA|nucA
- Application:
- ELISA, WB
- Concentration:
- 1.06 mg/ml
- Physical State:
- Liquid
- Storage Conditions:
- -20[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles., 2-8[o]C diluted. Aliquot. Avoid freeze/thaw cycles.
- Supplier:
- Rockland Inc
- Host:
- Rabbit
- Reactivities:
- Bacteria
- Buffer:
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
- Immunogen:
- Anti-Nuclease/NucA was prepared by repeated immunizations with recombinant benzonase protein produced in E. coli.
- Formulation:
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- Uniprot:
- P13717
- Synonyms:
- C7M65_14040;C7M65_RS14055;DNA/RNA non-specific endonuclease;Endonuclease
- Extra Details:
- Nucleases are secreted by Serratia marcescens into the medium it surrounds. The enzyme is a sugar-nonspecific hydrolase, capable of cleaving both RNA and DNA in either double or single stranded form. It requires divalent cations, preferably Mg2+, and is functional across a broad pH range from 6 to 10 (optimal at 8-8.5) and wide temperature ranges between 35°C and 44°C. The ability of Serratia to secrete nuclease appears to be regulated. Bacterial cultures at differing cell densities display different kinetics and efficiencies of nuclease secretion [i.e. growth medium, growth conditions, and host cell mutations]. Anti-Nuclease/NucA Antibody is useful for researcher interested in identifying nucleic acid contamination.
- Shipping Conditions:
- Dry Ice