200-401-A51
SARS 3Cl Protease Antibody
Cannot supply to this region.
- SKU:
- 200-401-A51
- Additional Names:
- 3CL-PRO|rabbit anti-Sars CoV 3CL Protease Antibody, 3CL PRO antibody, 3CLp antibody, nsp5 antibody
- Application:
- ELISA, WB, IF
- Concentration:
- 5.1 mg/ml
- Physical State:
- Lyophilized
- Species Reactivity:
- Virus
- Storage Conditions:
- 2-8[o]C liquid. Aliquot. Avoid freeze/thaw cycles., -20[o]C/-70[o]C aliquoted. Aliquot. Avoid freeze/thaw cycles.
- Supplier:
- Rockland Inc
- Host:
- Rabbit
- Buffer:
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride
- Immunogen:
- This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a purified recombinant protein corresponding to full length SARS Coronavirus 3CL Protease. Lifesensors Inc. (www.lifesensors.com) prepared the 3CL Protease as follows: SUMO-3CL protease fusion was expressed in E. coli in LB medium and purified by Ni-NTA resin affinity chromatography (Qiagen). After the fusion was cleaved by the SUMO Protease (LifeSensors), the SUMO tag and protease were subtracted from the 3CL protease using MAC and the 3CL protease was finally purified using Anion Exchange Chromatography with the Macro-Prep High Q resin (BioRad) and size exclusion chromatography.
- Formulation:
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
- Uniprot:
- P0C6U8
- Synonyms:
- ORF1a polyprotein;ORF1a polyprotein;ORF1ab polyprotein;polyprotein pp1a;polyprotein pp1ab;replicase 1AB;sarsp1
- Extra Details:
- Generally, viruses have proteases to process their proteins into active form. Because of its pivotal role in the viral life cycle, proteases are primary targets for the development of antiviral agents. 3CL protease, a viral cysteine proteinase, plays an important role in co-translational proteolytic processing of Coronavirus polyproteins. The 3CL protease cleaves as much as 11 sites in the replicase polyproteins and also releases the key replicative functions of polymerase and helicase. 3CL protease is the only Coronavirus protein for which structural information is available. 3CL protease comprises three domains, the substrate-binding site is expected to be located between domains I and II, and domain III is a globular cluster comprising five helices. 3CL protease is a homodimer. Anti-SARS-CoV 3CL Protease Antibody is useful for researchers interested in viral research.
- Shipping Conditions:
- Ambient