985
Green Fluorescent FAM-Phe-DAP Serine Protease Assay Kit
Size
£658.00
- SKU:
- 985
- Application:
- Flow Cytometry
- Physical State:
- Cell Culture, Tissue
- Purification:
- Intracellular serine protease activity was monitored over time in Jurkat cells exposed to 1 uM staurosporine. Cells were treated with staurosporine for 1, 2, 3, and 4 hours to induce apoptosis and increase serine protease activity (green histograms), or were untreated (gray histograms). Samples were stained with FAM-Phe-CMK (FFCK, Kit #946) for 1 hour at 37°C prior to being washed to remove unbound FLISP and then analyzed using an Attune NxT flow cytometer. The amount of serine protease activity detected correlated to the duration of the exposure period; the longer the cells were exposed to staurosporine, the larger the portion of serine protease positive cells found in the sample. Data courtesy of Dr. Kristi Strandberg (ICT 231:75-79).
- Storage Conditions:
- 2-8[o]C, -20[o]C
- Supplier:
- ImmunoChemistry Technologies
- Immunogen:
- Chymotrypsin-like enzymes
- Extra Details:
- Quantify intracellular active chymotrypsin-like serine protease activity in vitro. The DAP group phosphonylates the active site serine residue. Analyze your samples using a flow cytometer, fluorescence microscope, or fluorescence plate reader., ICT
- Shipping Conditions:
- Blue Ice