983
Red Fluorescent SR In vivo Poly (active) Caspase (VAD) Assay
Size
£653.00
- SKU:
- 983
- Application:
- Flow Cytometry
- Physical State:
- Tissue
- Purification:
- IN VIVO APOPTOSIS DETECTION, SR-FLIVO was used to assess the effectiveness of arsenic trioxide (ATO) treatment on SCK mammary tumors in vivo. Using a window chamber to view tumors directly, SCK mammary tumor cells were grown under the skin of A/J mice for 7 days. Test mice were treated with ATO and the control mice received a placebo (24 hours). All mice were injected IV with SR-FLIVO and images were captured 30 minutes later. The control SCK tumor (A.) exhibited a base level of apoptosis as expected (18%, FACS data not shown), while the ATO-treated tumor (B.) exhibited a much higher level of apoptosis (36%, FACS data not shown). Bright spots indicate high levels of caspase activity within the tumor. ATO treatment doubled the level of caspase activity. Data courtesy of Dr. Robert Griffin, University of Minnesota.
- Storage Conditions:
- 2-8[o]C
- Supplier:
- ImmunoChemistry Technologies
- Immunogen:
- Poly Caspases
- Extra Details:
- A non-cytotoxic, cell-permeant fluorescent inhibitor of caspases optimized for use in whole live animals. The probe contains the preferred binding sequence for most caspases (Val-Ala-Asp or VAD), coupled to a SR dye and a FMK reactive entity., ICT
- Shipping Conditions:
- Blue Ice