980
Green Fluorescent FAM In vivo Poly (active) Caspase (VAD) Assay
Size
£273.00
- SKU:
- 980
- Application:
- Flow Cytometry
- Physical State:
- Tissue
- Purification:
- CASPASE ACTIVITY IN RAT BRAIN, Using FAM-FLIVO to monitor cell death, there is a clear distinction between healthy and apoptotic neurons. In this live animal brain study of diabetes, Dr. Thomas Morrow at the University of Michigan VAMC Ann Arbor was able to assess neurodegeneration via caspase activity in control (left) and 8-week STZ diabetic rats (right). 30 minutes prior to sacrifice, FAM-FLIVO (catalog #981) was injected intravenously to directly label caspase-positive apoptotic neurons. After sacrifice, 20 um frozen sections of the periaqueductal gray (PAG) were prepared and counter-stained with red fluorescent Nissl to identify all neurons. Dying apoptotic neurons exhibit dual staining with FAM-FLIVO (yellow/green) and Nissl (red). In this model, diabetic animals show greater levels of caspase activity in the PAG than control animals.
- Storage Conditions:
- 2-8[o]C
- Supplier:
- ImmunoChemistry Technologies
- Immunogen:
- Poly Caspases
- Extra Details:
- A non-cytotoxic, cell-permeant fluorescent inhibitor of caspases optimized for use in whole live animals. The probe contains the preferred binding sequence for most caspases (Val-Ala-Asp or VAD), coupled to a FAM dye and a FMK reactive entity., ICT
- Shipping Conditions:
- Blue Ice