Skip to content

Download Spec Sheet

download
  1. Shop all
  2. Enzymes

960

Red Fluorescent SR-FLICA[R] Caspase-9 (LEHD) Assay Kit

Size

£285.00

SKU:
960
Application:
Flow Cytometry
Physical State:
Cell Culture
Purification:
Jurkat cells were treated with 1 uM staurosporine to induce caspase-9 activity (left), or a negative control (right) for 3 hours, washed twice, then incubated with ICT's red caspase-9 inhibitor probe, SR-LEHD-FMK, for 1 hour and examined under a fluorescence microscope. DIC images were also taken of all cells. Both induced images reveal several experimental cells which fluoresce red, therefore they have some degree of caspase-9 activity. The non-induced DIC image reveals many control cells, however the corresponding fluorescence image is dark; none of these cells have active caspase-9 (Dr. Brian W. Lee, ICT).
Storage Conditions:
2-8[o]C
Supplier:
ImmunoChemistry Technologies
Immunogen:
Caspase-9
Extra Details:
This in vitro assay employs the red fluorescent inhibitor probe SR-LEHD-FMK to label active caspase-9 enzyme in living cells. Analyze samples using fluorescence microscopy, a fluorescence plate reader, or flow cytometry., ICT
Shipping Conditions:
Blue Ice