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Green Fluorescent FAM-FLICA[R] Caspase-3/7 (DEVD) Assay Kit

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£285.00

SKU:: 93
Application:: Flow Cytometry
Physical State:: Cell Culture
Purification:: Normal (left) and keratoconus (right) corneal fibroblasts were treated with 200 uM H2O2 for 1 hr, washed, and allowed to recover for 1-3 hrs. The culture media was removed and replaced with 1X FAM-FLICA Caspase-3/7 (kit cat.# 93) solution (in culture media) at 300 ul/well for 1 hr. The cell layer was washed 3 times with 1X wash buffer; 300 ul wash buffer was added to keep the cells from drying. Keratoconus corneal fibroblasts treated with H2O2 (right) show a significant increase in caspase-3/7 activity compared to normal cells (left). Non-apoptotic cells are dark in background. Data courtesy of Dr. Cristina Kenney, M.D., Ph.D. Dept. of Ophthalmology, UC Irvine.
Storage Conditions:: 2-8[o]C
Supplier:: ImmunoChemistry Technologies
ABP:: IMP-GEN-2004-10 -> Test Kits
Immunogen:: Caspase-3/7
Extra Details:: This in vitro assay employs the green fluorescent inhibitor probe FAM-DEVD-FMK to label active caspase 3 and caspase 7 enzymes in living cells. Analyze samples using fluorescence microscopy, a fluorescence plate reader, or flow cytometry., ICT