924
JC-1 Fluorescent Mitochondrial Membrane Depolarization Assay
Size
£296.00
- SKU:
- 924
- Application:
- Flow Cytometry
- Physical State:
- Cell Culture
- Purification:
- Healthy cells fluoresce red, dying cells fluoresce green. Jurkat cells were stained with ICT's MitoPT JC-1 dye and analyzed with a fluorescence microscope containing a long bandpass filter (excitation at 490 nm and emission >510 nm). In non-apoptotic healthy cells (2 cells at left), the reagent aggregates inside intact mitochondria and fluoresces red. As the mitochondrial membrane potential drops and cells enter apoptosis, the dye disperses throughout the cell. The reagent then assumes a monomeric form and fluoresces green (3 cells on right). Using a flow cytometer to analyze cells labeled with MitoPT JC-1, the instrument will measure apoptosis by monitoring the amount of red fluorescence in each region. Healthy cells with intact mitochondria fluoresce red due to aggregated MitoPT JC-1 and appear in R2. As the mitochondrial membrane potential collapses and cells enter apoptosis, MitoPT JC-1 reagent is dispersed throughout the cell, converting to its green fluorescent monomeric form. The amount of red fluorescence drops as these cells enter R3. In this example, Jurkat cells were either treated with DMSO (negative, non-induced cells) or with staurosporine (apoptotic, induced cells) for 4 hours and then labeled with MitoPT JC-1 for 15 minutes. Using a fluorescence plate reader, healthy cells will give a high OD reading of red fluorescence; apoptotic cells will generate a lower reading of red fluorescence. In this example, Jurkat cells were either treated with DMSO (negative, non-induced cells) or with staurosporine (apoptotic, induced cells) for 4 hours and then labeled with MitoPT JC-1 for 15 minutes. As the mitochondrial membrane potential collapsed, indicating apoptosis, the amount of red fluorescence dropped by half (51%) in Jurkat cells from 167 RFU to 82.
- Storage Conditions:
- -20[o]C
- Supplier:
- ImmunoChemistry Technologies
- Immunogen:
- Mitochondrial depolarization
- Extra Details:
- JC-1 accumulates and fluoresces orange in polarized mitochondria. Following membrane depolarization, JC-1 disperses within the cell and fluoresces green. Analyze using a flow cytometer, fluorescence plate reader, or fluorescence microscopy., ICT
- Shipping Conditions:
- Blue Ice