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Red Fluorescent SR-FLICA[R] Poly (active) Caspase (VAD) Assay Kit

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£285.00

SKU:: 916
Application:: Flow Cytometry
Physical State:: Cell Culture
Purification:: Jurkat cells were treated with 1 uM staurosporine to induce caspase activity (top), or a negative control (bottom) for 3 hours, incubated with ICT's red poly caspases inhibitor probe, SR-VAD-FMK, for 1 hour, washed twice, and examined under a fluorescence microscope (DIC images were also taken). The color image of induced cells (upper left) reveals experimental cells which fluoresce red, therefore they all have some degree of caspase activity. The non-induced DIC image (lower right) reveals many control cells, however the corresponding fluorescence image (lower left) is dark; none of these cells have active caspases (Dr. Brian W. Lee, ICT).
Storage Conditions:: 2-8[o]C
Supplier:: ImmunoChemistry Technologies
Immunogen:: Poly caspases
Extra Details:: This in vitro assay employs the red fluorescent inhibitor probe SR-VAD-FMK to label active caspase enzymes in living cells. Analyze samples using fluorescence microscopy, a fluorescence plate reader, or flow cytometry., ICT