9157
Autophagy Assay - Red Fluorescent
Size
£515.00
- SKU:
- 9157
- Application:
- Flow Cytometry
- Physical State:
- Cell Culture
- Purification:
- Jurkat cells were treated with 0.5 uM Rapamycin and 10 uM Chloroquine (red histogram) to induce autophagy or were mock treated (black histogram) for 18 hours. Cells were stained with Autophagy Probe, Red for 1 hour at 37°C, washed, and then analyzed using a BD Fortessa Special Order Flow Cytometer equipped with a green/yellow laser (561 nm excitation) and a 610/20 emission filter. Median fluorescence intensity was 3-fold greater in induced cells (10,530) compared to mock-treated cells (3, 497). Data courtesy of Dr. Strandberg, ICT, 228:16-20.
- Storage Conditions:
- 2-8[o]C, -20[o]C
- Supplier:
- ImmunoChemistry Technologies
- Immunogen:
- Autophagy
- Extra Details:
- For the detection and monitoring the in vitro development of autophagy in living cells. The autophagy probe fluoresces red when inserted in the lipid membranes of autophagosomes and autolysosomes. Results can be read using a flow cytometer., ICT
- Shipping Conditions:
- Blue Ice