9155
Nitric Oxide Synthase Assay
Size
£286.00
- SKU:
- 9155
- Application:
- Flow Cytometry
- Physical State:
- Cell Culture
- Purification:
- Analysis via Flow Cytometry Jurkat cells were stained with ICT's DAF-2DA dye (Kit #9155) for 1 hour, washed, and then mock-treated with DMSO to create a negative control (left histogram) or 1 mM DEA NONOate, a nitric oxide donor (middle histogram), for 30 minutes at 37°C. Cells were read on the FL1 channel of an Accuri C6 flow cytometer. The median fluorescence intensity (MFI) of stained cells in the negative control was 324,817 in FL1-A (left : Negative), whereas the treated population had a value of 1,190,365 (middle: Positive), which is a increase of more than 3.5-fold. The effect of DEA NONOate on intracellular NOS activity is easily visible when the samples are overlaid in a single plot (right, black: Negative; right, red: Positive). Data courtesy of Dr. Kristi Strandberg, ICT, 227:76. Fluorescence microscopy and fluorescent plate reader data can be found in the Nitric Oxide Synthase Assay Manual.
- Storage Conditions:
- 2-8[o]C
- Supplier:
- ImmunoChemistry Technologies
- Immunogen:
- Nitric Oxide, NOS
- Extra Details:
- Assess the potency of nitrosative stress inhibitor and activator reagents. Measure the overall intracellular levels of free nitric oxide and NOS using a DAF-2DA dye. Analyze on a flow cytometer, fluorescence plate reader, or fluorescence microscope., ICT
- Shipping Conditions:
- Blue Ice