9148
Necrosis vs Apoptosis Assay Kit
Size
£726.00
- SKU:
- 9148
- Application:
- Flow Cytometry
- Physical State:
- Cell Culture
- Purification:
- Jurkat suspension cells were exposed to 1 uM of staurosporine for 4 hours at 37°C to induce apoptosis. Cells were dually stained with the green fluorescent FAM-FLICA poly caspase probe to detect apoptosis via caspase activity, and the red fluorescent vital dye 7-AAD to detect necrosis. The image shown in panel A reveals 4 populations of cells: 1) Live, unstained cells, which do not fluoresce. 2) Early stage apoptotic cells fluoresce green with FAM-FLICA. 3) Dually stained green and red fluorescing cells represent the population of Jurkat cells in mid-to-late stage apoptosis; these cells have active caspase enzymes and compromised cell membranes. 4) Necrotic cells fluoresce red. Panel B shows a corresponding differential interference contrast (DIC) image, which reveals cell morphology. Flow cytometry analysis of Jurkat suspension cells to quantify four populations. (A) Cells were treated with a placebo (non-induced treatment with DMSO). (B) Cells were treated with 1 uM staurosporine for 4 hours to induce apoptosis via caspase activity. Cells were then dually stained with FAM-FLICA and 7-AAD, and analyzed using an Accuri C6 flow cytometer. FAM-FLICA was analyzed on FL-1 and 7-AAD was analyzed on FL-3. The key is shown in (C). Live, unstained cells do not fluoresce (lower left quadrant). Early apoptotic cells fluoresce green with FAM-FLICA. Dually stained green and red fluorescing cells represent the population of cells in mid to late apoptosis (these cells have active caspase enzymes and compromised cell membranes). Necrotic cells fluoresce red. In the non-induced population (A), only 9.8% of cells were apoptotic (LR: 4.5% + UR: 6.3%), compared with 97.1% of the induced population (B; LR: 46.4% + UR: 50.7%).
- Storage Conditions:
- 2-8[o]C
- Supplier:
- ImmunoChemistry Technologies
- Immunogen:
- Caspases, GC-rich DNA
- Extra Details:
- This kit simultaneously detects both cell death due to both apoptosis and necrosis. It can be used to assess the effects of novel therapeutic agents on cell health. Analyze the fluorescent signal using fluorescence microscopy or flow cytometry., ICT
- Shipping Conditions:
- Blue Ice