9146
Pyroptosis/Caspase-1 Assay - Green Fluorescent
Size
£762.00
- SKU:
- 9146
- Application:
- Flow Cytometry
- Physical State:
- Cell Culture, Tissue
- Purification:
- THP-1 suspension cells were treated with PMA (5 ng/mL) to induce differentiation into macrophages. After 48 hours, PMA was removed and replaced with fresh medium that was spiked with LPS (10 ng/mL) to induce caspase-1 activation. After 2 hours, cells were stained with FAM-YVAD-FMK for 1 hour, washed, and examined under a fluorescence microscope. The top images reveal many experimental cells, several of which fluoresce green, therefore they have active caspase-1. The lower right image reveals many control cells in the field of view, however the corresponding fluorescence image is dark (lower left); few of these cells have active caspase-1. Data courtesy of Dr. Brian Lee, ICT.
- Storage Conditions:
- 2-8[o]C, -20[o]C
- Supplier:
- ImmunoChemistry Technologies
- Immunogen:
- Caspase 1
- Extra Details:
- This Pyroptosis/Caspase-1 Assay Kit utilizes our FLICA® technology to assess pyroptotic cells by detecting caspase-1 activation. Analyze the green fluorescent signal using fluorescence microscopy, a fluorescent plate reader, or by flow cytometry., ICT
- Shipping Conditions:
- Blue Ice