9144
Intracellular Total ROS Activity Assay
Size
£421.00
- SKU:
- 9144
- Application:
- Flow Cytometry
- Physical State:
- Cell Culture
- Purification:
- Jurkat cells were stained with ICT's Intracellular Total ROS Activity Assay (kit #9144) for 1 hour, and then treated with a negative control (left histogram) or 100 uM tert-Butyl hydroperoxide, a reactive oxygen-inducing agent used to create the positive control (middle histogram), for 30 minutes at 37°C. Cells were read on the FL1 channel of an Accuri C6 flow cytometer. The median fluorescence intensity (MFI) of stained cells in the negative control was 11,875 in FL1-A (left: Negative), whereas the treated population had a MFI value of 419,067 (middle: Positive), which is an increase of more than 35-fold. The effect of tert-Butyl hydroperoxide on intracellular total ROS activity is easily visible when the samples are overlaid in a single plot (right-most histogram, black: Negative; right, red: Positive). Data courtesy of Dr. Kristi Strandberg, ICT, 224:76.
- Storage Conditions:
- 2-8[o]C, -20[o]C
- Supplier:
- ImmunoChemistry Technologies
- Immunogen:
- ROS
- Extra Details:
- The Intracellular Total ROS Activity Assay enables the screening of oxidative stress inhibitor and activator reagents for potency. The assay assesses the overall level of intracellular ROS activity. Analyze using a flow cytometer., ICT
- Shipping Conditions:
- Blue Ice