9137
Intracellular GSH Assay Kit
Size
£310.00
- SKU:
- 9137
- Application:
- Flow Cytometry
- Physical State:
- Cell Culture
- Purification:
- Measurement of Intracellular GSH Levels in Non-Induced and Induced Jurkat cell samples. Jurkat cells were treated with a negative control agent or staurosporine to induce oxidative stress. Samples were then stained with the ThioBright™ GSH quantitation dye for 30 minutes per the Intracellular GSH Assay protocol. Flow cytometric analysis reveals a 30% decrease in median fluorescence intensity (MFI) with staurosporine treatment, corresponding to a decrease in intracellular GSH levels. The effect of staurosporine on intracellular GSH levels is easily visible when the samples are overlaid in a single plot (green: negative control; red: positive control). Data courtesy of Ms. Tracy Hanson, ICT.
- Storage Conditions:
- -20[o]C
- Supplier:
- ImmunoChemistry Technologies
- Immunogen:
- Glutathione (GSH)
- Extra Details:
- This whole cell glutathione assay employs a proprietary thiol-sensitive dye, to monitor relative changes in the intracellular concentration of reduced glutathione (GSH). Analyze the fluorescent signal by flow cytometry., ICT
- Shipping Conditions:
- Blue Ice