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Far-Red Fluorescent FLICA[R] 660 Poly (active) Caspase (VAD) Assay Kit

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£330.00

SKU:: 9120
Application:: Flow Cytometry
Physical State:: Cell Culture
Purification:: To demonstrate the fluorescence properties and apoptosis detection capability of the new far-red fluorescent FLICA 660 Poly Caspase Assay, Jurkat cells were were treated with 1 uM staurosporine for 4 hours and labeled with FLICA 660-VAD-FMK poly caspase inhibitor reagent. Using a Nikon E800 Hyperspectral Microscope equipped with a Photometrics HQ2 camera, far-red fluorescence emission from apoptotic Jurkat cells was acquired with a Cy5 HYQ bandpass filter combination set. FLICA 660-VAD-FMK reagent optimally excites at 660 nm and has an emission max at 690 nm, making it suitable for use with red lasers on common benchtop flow cytometers and ideal for dual staining purposes with green fluorescent probes. Composite overlay of DIC image and far-red fluorescence emission from apoptosis-induced Jurkat cells stained with FLICA 660 Poly Caspase Assay.
Storage Conditions:: 2-8[o]C
Supplier:: ImmunoChemistry Technologies
Immunogen:: Poly caspases
Extra Details:: This in vitro assay employs the far-red fluorescent inhibitor probe 660-VAD-FMK to label active caspase enzymes in living cells. Analyze the fluorescent signal using fluorescence microscopy or flow cytometry., ICT