9105
TMRM - Orange Fluorescent Mitochondrial Membrane Depolarization Assay
Size
£366.00
- SKU:
- 9105
- Application:
- Flow Cytometry
- Physical State:
- Cell Culture
- Purification:
- Jurkat cells were cultured in the absence (healthy) or presence (dying) of staurosporine to induce apoptosis and stained with ICT's potentiometric dye TMRM. Cells were analyzed using a Nikon E800 photomicroscope using a green excitation filter (510-560 nm) in tandem with a 570-620 nm emission filter. Apoptotic cells with depolarized mitochondria exhibit reduced orange/red fluorescence.
- Storage Conditions:
- -20[o]C
- Supplier:
- ImmunoChemistry Technologies
- Immunogen:
- Mitochondrial depolarization
- Extra Details:
- TMRM accumulates and fluoresces orange in polarized mitochondria. Fluorescence drops dramatically on depolarization in apoptotic or metabolically stressed cells. Analyze using a flow cytometer, fluorescence plate reader, or fluorescence microscopy., ICT
- Shipping Conditions:
- Blue Ice