Streptavidin (FITC)

Cannot supply to this region.

SKU:: GTX85911
Application:: Flow Cytometry, IHC-P, IF, ICC
Physical State:: Liquid
Storage Conditions:: 2-8[o]C Protect from light. Store undiluted.
Supplier:: Genetex
Buffer:: 10mM Phosphate, 150mM NaCl, 1% BSA, 0.05% Sodium azide.
Extra Details:: Streptavidin is a 55 kDa (subunit MW 14 kDa) biotin-binding protein isolated from Streptomyces avidini. Streptavidin is superior to avidin, because it does not contain carbohydrate like avidin and has no net charge at neutral pH. Streptavidin~biotin system is routinely used in Immunohistochemistry (IHC). Extinction Coefficient 1% A280=32.0. Purity: > 99% pure by HPLC; Eletrophoretically homogenous, single band. Protein content: > 95%; Activity 14.8 units/ mg, one unit bunds 1.0 microgram of d-biotin; Protease activity: 0.000018 units/mg; Isoelectric point: 7.0 +/- determined by Isoelectric focusing. One mg of Streptavidin is conjugated with FITC, unconjugated Streptavidin is removed. This conjugate of streptavidin is recommended for use with Biotin~conjugated antibodies. Avidin~Biotin conjugated reagents are stable, sensitive and give less background. Fluorophore: Fluorescein isothiocyanate A max=492nm; E=520nm; Fluorophore/Protein: A492nm/A280nm=~1