Streptavidin (APC)

Cannot supply to this region.

SKU:: GTX85908
Application:: Flow Cytometry, IF, ICC
Physical State:: Liquid
Storage Conditions:: 2-8[o]C Protect from light. Store undiluted.
Supplier:: Genetex
Buffer:: 10mM Phosphate, 150mM NaCl, 1% BSA, 0.05% Sodium azide.
Extra Details:: Streptavidin is a 55 kDa (subunit MW 14 kDa) biotin-binding protein isolated from Streptomyces avidini. Streptavidin is superior to avidin, because it does not contain carbohydrate like avidin and has no net charge at neutral pH. Streptavidin~biotin system is routinely used in Immunohistochemistry (IHC). Extinction Coefficient 1% A280=32.0; Purity: Streptavidin used is 99% pure by HPLC; Eletrophoretically homogenous, single band, Protein content: > 95%; Activity 14.8 units/ mg, one unit bunds 1.0 microgram of d-biotin; Protease activity: 0.000018 units/mg; Isoelectric point: 7.0 +/- determined by Isoelectric focusing. Allophycocyanin is a protein from the light-harvesting phycobiliprotein family, along with phycocyanin, phycoerythrin and phycoerythrocyanin. It is an accessory pigment to chlorophyll. Allophycocyanin absorbs and emits red light (650 & 660?nm max, respectively), and is readily found in Cyanobacteria (also called blue-green algae), and red algae. Phycobilin pigments have fluorescent properties that are used in immunoassay kits. Allophycocyanin can be isolated from various species of red or blue-green algae, each producing slightly different forms of the molecule. It is composed of two different subunits (A Alpha and B Beta) in which each subunit has one phycocyanobilin (PCB) chromophore. The subunit structure for APC has been determined as (A AlphaB Beta)3. The molecular weight of APC is 105,000 Daltons. Absorption max: 652 nm, additional adsorption 625 nm, Emission max. 657.5 nm, Extinction Coefficient: 2.4 1 (mg/ml), A650/A280=4, A650/A620=1.25.

Shipping Conditions:: Blue Ice