GTX22845
Chk1 antibody
Cannot supply to this region.
- SKU:
- GTX22845
- Additional Names:
- checkpoint kinase 1 , CHK1
- Application:
- WB
- Physical State:
- Liquid
- Species Reactivity:
- Human
- Purification:
- Affinity Purified
- Storage Conditions:
- 2-8[o]C Do not freeze.
- Supplier:
- Genetex
- Host:
- Goat
- Reactivities:
- Human
- Buffer:
- Tris-Citrate/Phosphate, 0.09% Sodium azide.
- Immunogen:
- The immunogen this antibody was made to, maps to a region between residues 200 and 250 of human Checkpoint Kinase 1 using the numbering given in entry NP_001265.1 (GeneID 1111).
- Uniprot:
- O14757
- Synonyms:
- cell cycle checkpoint kinase;Checkpoint kinase-1;Checkpoint, S. pombe, homolog of, 1;CHK1;CHK1 checkpoint homolog;Chk1-S;serine/threonine-protein kinase Chk1
- Extra Details:
- Required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-279' and 'Ser-293' promotes proteolysis of CDC25A. Inhibition of CDC25 activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Binds to and phosphorylates RAD51 at 'Thr-309', which may enhance the association of RAD51 with chromatin and promote DNA repair by homologous recombination. Binds to and phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A. This may affect chromatin assembly during S phase or DNA repair. May also phosphorylate multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and enhances suppression of cellular proliferation.
- Shipping Conditions:
- Blue Ice