Human ACHE Protein; His Tag

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£178.00

SKU:: PME101227
Additional Names:: ACEE;ARACHE;N-ACHE;YT
Molecular Weight:: The protein has a predicted molecular mass of 65.4 kDa after removal of the signal peptide. The apparent molecular mass of ACHE-His is approximately 55-70 kDa due to glycosylation.
Purity:: The purity of the protein is greater than 85% as determined by SDS-PAGE and Coomassie blue staining.
Storage Conditions:: Store at -20[o]C to -80[o]C for 12 months in lyophilized form. After reconstitution; if not intended for use within a month; aliquot and store at -80[o]C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
Supplier:: DIMA Biotechnology Ltd
Formulation:: Lyophilized from sterile PBS; pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
Target:: ACHE
ACHE(Glu32-Leu614) 6×His tag:: ACHE(Glu32-Leu614) 6×His tag
Extra Details:: Acetylcholinesterase hydrolyzes the neurotransmitter; acetylcholine at neuromuscular junctions and brain cholinergic synapses; and thus terminates signal transmission. It is also found on the red blood cell membranes; where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties; but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene; and the structural diversity in the gene products arises from alternative mRNA splicing; and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain; muscle and other tissues is the hydrophilic species; which forms disulfide-linked oligomers with collagenous; or lipid-containing structural subunits. The other; alternatively spliced form; expressed primarily in the erythroid tissues; differs at the C-terminal end; and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally. AChE activity may constitute a sensitive biomarker of RBC ageing in vivo; and thus; may be of aid in understanding the effects of transfusion[provided by RefSeq; Sep 2019]

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