TrueBlot® For Optimised IP-WB Methods

Immunoprecipitation (IP) and Western blot (WB) protocols provide highly specific results but often suffer from poor or non-specific binding, high background, and heavy/light chain interference.

Rockland’s TrueBlot® line of products consists of both TrueBlot® IP beads and TrueBlot® monoclonal secondary antibodies, which combat these common experimental issues by providing increased specificity, low background, and enhanced accuracy to provide publication-quality IP Western blots without interference from contaminating IgG. 

The TrueBlot® product line is ideal for studying protein-protein interactions, post-translational modifications (phosphorylation and acetylation), poorly expressed proteins, and immunoprecipitated proteins with molecular weights similar to the heavy and light chain.

TrueBlot® secondary antibodies only recognize primary antibodies in their native (non-reduced) state, eliminating interference from heavy and light chain bands to provide accurate, publication-quality Western blots of immunoprecipitated samples. Pair TrueBlot® antibodies with the exceptional purification properties provided by TrueBlot® IP beads to eliminate the common issues of IP-Western Blot.