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Introduction to DNA Design for myTXTL

Learn the important elements required for the design of DNA templates used in the myTXTL system for either plasmid or linear DNA templates.

myTXTL™ is an E.coli based protein expression system to enable cell free protein transcription translation – an in vitro protein translation and transcription kit (I.V.T.T.)

 

The advantages of an IVTT kit is the speed at which proteins can be produced – without having to optimise cellular growth conditions and transfection conditions. Some proteins can be difficult to express in live cells, since they can affect cellular metabolism – such as toxic proteins.  

 

With IVTT kits, designed proteins can be made with a synthetic DNA to see if the A.I. predictions are accurate, or bacteriophages can be re-booted, and can eliminate the need for phage screening and subsequent cloning.

As the system is based on bacterial cellular machinery, variations of the myTXTL™ kits include supplements to help protein folding for more complicated mammalian proteins like antibodies.

The short video runs through the requirements for the DNA template to use with the myTXTL™ system -   once you have your DNA and a myTXTL™ kit, you’re ready to start expressing protein!

 

If you would like to discuss your protein expression experiment, please get in touch with our Product Managers!

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