Human Cell Lines from Cytion
Human Cell Lines
Discover a comprehensive portfolio of authenticated human cell lines from Cytion, designed to support cutting-edge biomedical and pharmaceutical research. Each cell line is rigorously tested for identity, purity, and contamination to ensure reliable and reproducible results in your laboratory.
Cytion offers a wide selection of human cell models derived from different tissues and diseases, providing researchers with dependable tools for studying disease mechanisms, drug discovery, protein production, and vaccine development.
Cells that have been immortalised and grown in vitro from primary explants of human tissue or bodily fluid are referred to as a human cell line.
Key Features
- Authenticated and validated cell lines ensuring genetic integrity and reproducibility
- Mycoplasma-free and contamination-tested for dependable experimental outcomes
- Extensive portfolio covering numerous cancer types and biological models
- Suitable for multiple applications including drug discovery, virus research, and protein production
- Reliable supply with consistent quality standards
Immortality of human cell lines
Cost-effective, user-friendly, and able to undergo more passes than primary cells, cell lines are preferred by scientists. Cell lines are simple to manipulate and propagate, making them preferred for numerous screenings due to the benefit of a limitless supply of materials.
Cells that have been immortalised can be cultivated forever when their growth has been artificially stimulated. Different types of cancers and other cells with chromosomal defects or mutations that allow them to proliferate indefinitely provide the basis for immortalised cell lines.
As a result of their rapid proliferation, the dish or flask containing immortalised cells will become overcrowded. That is why scientists create more space for proliferating cells by passaging (or dividing) onto fresh plates.
Applications
Cytion human cell lines are widely used in:
- Cancer biology research
- Drug screening and therapeutic development
- Recombinant protein expression
- Vaccine and virus research
- Molecular and cellular biology studies
Human cell lines provide essential insights into biological systems and disease mechanisms while enabling scalable laboratory research and reproducible experiments.
Methods for generating immortal cells
Spontaneous mutation
During the process of cell division and multiplication, certain initial cells may be altered and exceed their lifetime. These cells will be harvested for expanded cell culture and will undergo spontaneous mutation to become immortalisation cells. In most instances, however, the cells will change into tumour cells, rendering this technique ineffective. Therefore, tumour cells are the finest example of spontaneously immortalised cells, which may have acquired genetic modifications to survive senescence and become immortal.
Inducing cell immortality by virus genes
Numerous viral genes can influence the cell cycle, allowing them to achieve immortality by eliminating the biological brakes on proliferative regulation. To promote immortalisation, the simian virus 40 (SV40) T-antigen is one way. It has been shown that SV40 T-antigen is the simplest and most dependable agent for the immortalisation of several cell types, and its mechanism in cell immortalisation is well known. An example is the cell type HEK293T (also known as 293T).
TERT/ Telomerase Reverse Transcriptase Protein Expression
Telomerase is a ribonucleoprotein that may prolong the DNA sequence of telomeres, therefore prevent cellular senescence and allowing cells to divide indefinitely. This protein is inactive in most somatic cells, but when TERT is produced exogenously, the cells are able to maintain enough telomere lengths to prevent replicative senescence. Currently, human telomerase reverse transcriptase (hTERT) is the most used method for cell immortalisation.
Explore Human Cell Line Categories
Cytion maintains a large and continuously expanding cell bank, offering hundreds of well-characterised human cell lines from diverse tissues and disease backgrounds.
Leukaemia/lymphoma | Liver Cancer | ||
Breast cancer | Brain Cancer | Hybrid/ immortalized Cells | Lung cancer |
Product Highlights
Why Choose Cytion Human Cells?
Cytion is a trusted provider of high-quality cell culture solutions, offering rigorously tested and authenticated cell lines that help researchers achieve reliable and reproducible results. With strict quality control processes and a broad portfolio, Cytion supports laboratories worldwide in advancing scientific discovery.
The Advantages of Human Cell Lines
- Consistency and Reproducibility: Human cell lines are well-defined and uniform, aiding in consistent and reproducible results.
- Ease of Cultivation: Easier to cultivate than primary cells, requiring no tissue extraction.
- High Protein Production: Capable of producing large protein quantities for assays.
- Genetic Modification: Can be modified to express specific genes, useful for research.
The Disadvantages of Human Cell Lines
- Limited Representation: May not accurately represent normal in vivo cell conditions.
- Genetic Drift: Genetic drift can occur over time, altering cell characteristics.
- Alteration Over Time: Extended passaging can lead to loss of original cell features.
- Reduced Physiological Relevance: Physiological relevance to human conditions may be reduced.
- Need for Validation: Requires careful validation to ensure authenticity and purity.
