1906
FLAT tagged CRISPR/Cas9 nuclease Adenovirus (Ad-mCherry-FLAG-hCas9)
Size
£882.00
- SKU:
- 1906
- Additional Names:
- Ad-mCherry-FLAG-hCas9|FLAT tagged CRISPR/Cas9 nuclease Adenovirus
- Concentration:
- 1E10 PFU/ml
- Storage Conditions:
- -70[o]C Avoid freeze/thaw cycles.
- Supplier:
- Vector Biolabs
- Buffer:
- DMEM, 2% BSA, 2.5% Glycerol
- Species:
- Virus
- Extra Details:
- Cas9 is the nuclease guided by the crRNA and tracrRNA (or trans-activating crRNA) to cleave specific DNA sequences. A guide RNA (gRNA) can be designed to include a hairpin that mimics the tracrRNA-crRNA complex. Binding specificity is based on the gRNA and a three nucleotide NGG sequence called the protospacer adjacent motif (PAM) sequence. With its ease in designing guide sequences to target specific genomic loci, the CRISPR/Cas system is a much simpler, faster, and robust alternative to TALEN and Zinc finger nuclease platforms. For site-specific genome editing, the CRISPR/Cas9 system requires the Cas 9 nuclease and the gRNA. This adenovirus expresses a codon-optimized Cas9 nuclease, along with a mCherry reporter. It can be used along with guided RNA for genome-editing.This adenovirus expresses a FLAG tagged Cas9 with a mCherry reporter., vectorbiolabs
- Shipping Conditions:
- Dry Ice