FLAT tagged CRISPR/Cas9 nuclease RGD-fiber modified Adenovirus (Ad(RGD)-GFP-FLAG-hCas9)

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£882.00

SKU:: 1905
Additional Names:: Ad(RGD)-GFP-FLAG-hCas9|FLAT tagged CRISPR/Cas9 nuclease RGD-fiber modified Adenovirus
Concentration:: 1E10 PFU/ml
Storage Conditions:: -70[o]C Avoid freeze/thaw cycles.
Supplier:: Vector Biolabs
Buffer:: DMEM, 2% BSA, 2.5% Glycerol
Species:: Virus
Extra Details:: The Type II prokaryotic CRISPR/Cas system is the new class of tools for targeted genome engineering. The cas9 nucleases derived from clustered regularly interspaced short palindromic repeats (CRISPR)-cas systems uses small RNAs as guides (gRNA) to cleave DNA in a sequence-specific manner. With its ease in designing guide sequences to target specific genomic loci, the CRISPR/Cas system is a much simpler, faster, and robust alternative to TALEN and Zinc finger nuclease platforms. For site-specific genome editing, the CRISPR/Cas9 system requires the Cas 9 nuclease and the gRNA. This RGD-fiber modified adenovirus expresses a codon-optimized spCas9 nuclease with FLAG tag and a GFP reporter. It can be used along with guided RNA for genome-editing in cells that regular Ad5 doesn't infect well, including human or mouse macrophages, ES cells, T cells, synoviocytes, certain fibroblast and islet grafts etc., vectorbiolabs