1776
codon-optimized FLP (FLPo) and eGFP Adenovirus (Ad-GFP-FLPo)
Size
£654.00
- SKU:
- 1776
- Additional Names:
- Ad-GFP-FLPo|codon-optimized FLP (FLPo) and eGFP Adenovirus
- Concentration:
- 1E10 PFU/ml
- Storage Conditions:
- -70[o]C Avoid freeze/thaw cycles.
- Supplier:
- Vector Biolabs
- Buffer:
- DMEM, 2% BSA, 2.5% Glycerol
- Species:
- Virus
- Extra Details:
- FLP from Saccharomyces cerevisiae, a site-specific recombinases (SSR) from the I>> integrase family, recognizes distinct 34 bp FRT sites. It has been widely used for generating conditional knockout mice, mediated by FLP/FRT system. Initial use of FLP in mammalian cells revealed inefficient recombinase activity due to thermo-instability of the FLP protein. Subsequent screening for thermo-stable mutants resulted in the identification of FLPe, with a 4-fold increase in recombination efficiency at 37C. However the recombination efficiency of FLPe in cells remains very low because of its non-mammalian origin. This mouse codon optimized FLPe greatly increase the recombination efficiency in mouse cells.This adenovirus express a mouse codon-optimized FLP recombinase (FLPo), along with an eGFP marker. FLPo and eGFP are driven by the same CMV promoter and separated by a 2A peptide., vectorbiolabs
- Shipping Conditions:
- Dry Ice