ZO-1 (Tight junction protein 1, Tight junction protein ZO-1, ZO1, ZO-1, Zona Occludens 1 Protein, Zonula Occludens 1 Protein)

Size

£642.00

SKU:: Z0750-02F
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Synthetic peptide corresponding to C-PKPTSQNQFSEHDKT, from the internal region of human ZO-1 (NP_003248.3; NP_783297.2).
Format:: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Extra Details:: ZO-1 is a protein located on a cytoplasmic membrane surface of intercellular tight junctions. It may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for this gene. Applications: Suitable for use in ELISA and Western Blot. Other applications have not been tested. Recommended Dilutions: ELISA: 1:128,000 Western Blot: 0.3-1ug/ml detects an ~170kD band observed in human brain (cerebral cortex) lysates (calculated MW of 187kD according to NP_783297.2). Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.