Z0300-05A
ZAP 70 (TCR Zeta Associated Protein kinase 70kD) (FITC)
Size
£793.00
- SKU:
- Z0300-05A
- Purity:
- Purified by Protein G affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- GST-fusion protein containing the 2 tandem SH2 domains of human ZAP-70 corresponding to aa1-254. Species sequence homology: rat
- Clone:
- 2F3.2
- Format:
- Supplied as a liquid in PBS, 0.05% sodium azide. Labeled with Fluorescein isothiocyanate (FITC).
- Extra Details:
- ZAP70, a 70kD member of the Syk tyrosine kinase family, plays a central role in lymphocyte activation and development, and is implicated in several immune disorders. Mutations in ZAP70 gene results in a form of Severe Combined Immunodeficiency Syndrome (SCID) in humans. ZAP70 expression also defines a subset of Chronic Lymphocytic Leukemia (CLL) in patients with unmutated Ig gene and poor clinical course. Applications: Suitable for use in Immunocytochemistry, Flow Cytometry and Western Blot. Other applications not tested. Recommended Dilution: Immunocytochemistry: 5-20ug/ml detected ZAP-70 in Jurkat cells fixed with formaldehyde and permeabilized with saponin. Western Blot: 1:500 detected ZAP 70 on 10ug of Jurkat lysates Optimal dilutions to be determined by the researcher. Positive Control: Western Blot: Jurkat cell lysate. Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice