X9505-13-ML490

XRCC5 (X Ray Repair Complementing Defective Repair in Chinese Hamster Cells 5 (Double-strand-break Rejoining), X-ray Repair Cross-complementing Protein 5, 86kD Subunit of Ku Antigen, ATP Dependent DNA Helicase 2 Subunit 2, ATP-dependent DNA Helicase II, ATP-dependent DNA Helicase II 80kD Subunit, CTC Box Binding Factor 85kD, CTC85, CTCBF, DNA Repair Protein XRCC5, FLJ39089, G22P2, Ku 80, Ku80, Ku Autoantigen 80kD, Ku 86, Ku86, Ku86 Autoantigen-related Protein 1, KARP1, KARP-1, KUB2, Lupus Ku Autoantigen Protein p86, Nuclear Factor IV, NFIV, Thyroid Lupus Autoantigen, TLAA, EC 3.6.1.-, Double Strand Break Rejoining, X-ray Repair Complementing Defective Repair in Chinese Hamster Cells 5) (Azide Free) (MaxLight 490)

Size

£914.00

SKU:: X9505-13-ML490
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: 4°C Do Not Freeze
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to a portion of amino acids within aa1-733 of human XRCC5.
Clone:: 3D8
Format:: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.
Extra Details:: MaxLight™490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection. Absorbance (491nm); Emission (515nm); Extinction Coefficient 73,000. The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependent DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. [provided by RefSeq] Applications: Suitable for use in FLISA, Immunohistochemistry (paraffin), and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:1,000 Immunohistochemistry (paraffin): 1.5ug/ml FLISA: 1ug/ml-3ng/ml Optimal dilutions to be determined by the researcher. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight™490 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note: Applications are based on unconjugated antibody.

Shipping Conditions:: Blue Ice