X9501-01X
XRCC1 (X-Ray Repair Cross Complementing Protein1) (BSA & Azide Free)
Size
£1,151.00
- SKU:
- X9501-01X
- Purity:
- Purified by Protein A affinity chromatography.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Mouse
- Immunogen:
- Recombinant human XRCC1 protein
- Clone:
- 33–2–5
- Format:
- Supplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See X9501-01
- Extra Details:
- X-Ray Repair Cross Complementing (XRCC1) plays a role in excision repair of DNA after ionizing irradiation. XRCC1 binds to DNA ligase III through the C-terminal 96 amino acids and to DNA polymerase beta through the N-terminal half.. In testis XRCC1 is expressed at high levels. Cells with a mutation of this gene exhibit decreased single strand break repair and reduced recombination repair. They show increased double strand breaks, and sister chromatid exchange is increased up to 10-fold. The XRCC1 might serve as a scaffold protein during base excision-repair. MW of Antigen: 85kD Epitope: Not determined Applications and Recommended Dilutions: Immunoprecipitation (Native verified) (Use Protein A) (Ab 2ug/mg protein lysate) Western Blotting (Ab 1-2ug/ml for 2hrs at RT) Immunohistochemistry (Formalin/paraffin) (1-2ug/ml for 30 min at RT; is better) * [Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.] The optimal dilution for a specific application should be determined by the investigator. Positive Control: Ls174T, HT29, or HeLa cells. Normal testis. Cellular Localization: Nuclear Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
- Shipping Conditions:
- Blue Ice