X1048-11

XPG (Xeroderma pigmentosum Type G, ERCC5, ERCM2, Excision Repair Cross Complementing Rodent Repair Deficiency, UVDR, Xeroderma Pigmentosum Complementation Group G, XPGC)

Size

£927.00

SKU:: X1048-11
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: 4°C (-20°C Glycerol)
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Purified Human recombinant XPG protein
Clone:: 3H55
Format:: Supplied as a liquid in PBS, pH 7.4, 0.1% BSA, 0.09% sodium azide, 40% glycerol.
Extra Details:: Xeroderma pigmentosum (XP) is characterized high sensitivity to UV light and high incidence of skin cancer. XP fall into seven complementation groups, XPA to XPG. They are lacking a functional helicase, endonuclease, or lesion-recognizing proteins involved base excision repair. Xeroderma pigmentosum type G (XPG) is one of the rarest and phenotypically heterogeneous forms of genetic disorder. XPG gene encodes an acidic protein with an estimated MW ~133kD that confers normal UV-resistance. XPG is a member of the flap endonuclease 1 (FEN-1) structure-specific DNA repair endonuclease family responsible to UV-induced DNA repair. Human XPG makes the 3' incision during nucleotide excision repair of DNA. The enzyme cleaves model DNA bubble structures specifically near the junction of unpaired DNA with a duplex region. A 29aa region of human XPG (981-1009aa) contains the PCNA binding activity. A conserved Arg in XPG (Arg992) is crucial for its PCNA-binding activity. Replication protein A (RPA) binds specifically and directly to two excision repair proteins, Xeroderma pigmentosum damage recognition protein XPA and the endonuclease XPG. Applications: Suitable for use in Western Blot, Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1-5ug/ml. Detects > 185kD from DiFi and other cells and tissues. Immunoprecipitation: 5-10ug/mg of protein lysate. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Shipping Conditions:: Blue Ice