X1044

XmiI (AccI)

Size

£346.00

SKU:: X1044
Storage Conditions:: -20°C
Supplier:: United States Biological
Extra Details:: A T 5'-G T^C G A C-3' 3'-C A G C^T G-5' T A Concentration: 10u/ul Unit Definition: One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 37°C in 50ul of assay buffer. Source: Xanthomonas maltophilia Jo 21–021 Buffer: 10mM Tris-HCl (pH 7.5), 10mM MgCl2 and 0.1mg/ml BSA. Incubate at 37°C. Diluent Buffer: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol, or Storage Buffer. Storage Buffer: 10mM Tris-HCl (pH 7.5 at 25°C), 100mM NaCl, 1mM DTT, 0.1mM EDTA, 0.2mg/ml BSA and 50% glycerol. Quality Control: Overdigestion Assay: No detectable change in the specific fragmentation pattern is observed after 160-fold overdigestion (10u/ug lambda DNA x 16 hours) with XmiI. Ligation/Recutting Assay: After 50-fold overdigestion (3u/ug DNA x 17 hours) with XmiI, more than 95% of the DNA fragments can be ligated at a 5'-termini concentration of 0.1uM. More than 95% of these can be recut. Labeled Oligonucleotide (LO) Assay: No detectable degradation of a single-stranded and double-stranded labeled oligonucleotide was observed after incubation with 10 units of restriction endonuclease for 4 hours. Blue/White Cloning Assay: pUC57 was digested at a unique site with 10 units of enzyme for 16 hours. After religation and transformation 0.2% of white colonies were detected. Methylation Effects: XmiI does not cut GTm5CGAC and GTMKAm5C. Blocked by overlapping CG methylation. Stability during Prolonged Incubation: A minimum of 0.1 units of enzyme is required for complete digestion of 1ug of lambda DNA in 16 hours at 37°C. Thermal Inactivation: Enzyme is inactivated by incubation at 65°C for 20min. Digestion of Agarose-embedded DNA: A minimum of 5 units of enzyme is required for complete digestion of 1ug of agarose-embedded lambda DNA in 16 hours. Compatible Ends: GT^CGAC–AciI, Bsp119I, Bsu15I, Hin1I, Hin6I, HpaII, MaeII, MspI, NarI, Psp1406I, TaqI. Number of Recognition Sites in DNA: Lambda: 9 PhiX174: 2 M13mp18/19: 1 pBR322: 2 pUC18/19: 1 pUC57: 1 pTZ19R/U: 1 pBluescriptIIKS(-/+): 1 pBluescriptIISK(-/+): 1 pACYC177: 1 pACYC184: 2

Shipping Conditions:: Blue Ice