XagI (EcoNI)

Size

£355.00

SKU:: X0960
Storage Conditions:: -20°C
Supplier:: United States Biological
Extra Details:: 5'-C C T N N^N N N A G G-3' 3'-G G A N N N^N N T C C-5' Source: Xanthobacter agilis Vs18-132 Supplied With: R1625: Restriction Enzyme Buffer A, 10X; (1X compostition after dilution)-33mM Tris-acetate, pH 7.9, 10mM magnesium acetate, 66mM potassium acetate, 0.1mg/ml BSA. R1625-04: Restriction Enzyme Buffer E, 10X: 10mM Tris-HCl, pH 8.5,10mM magnesium chloride, 100mM potassium chloride, 0.1mg/ml BSA Concentration: 10u/ul Unit Definition: One unit is defined as the amount of enzyme required to digest 1ug of lambda DNA in 1 hour at 37°C in 50ul of assay buffer. Diluent Buffer: 10mM Tris-HCl (pH 7.4 at 25°C), 100mM potassium chloride, 1mM EDTA, 1mM DTT, 0.2mg/ml BSA and 50% glycerol. Storage Buffer: 10mM Tris-HCl (pH 7.5 at 25°C), 100mM potassium chloride, 1mM DTT, 1mM EDTA, 0.2mg/ml BSA and 50% glycerol.