W3037-60
Wnt9b (Wnt-14b, Wnt-15, Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family Member)
Size
£812.00
- SKU:
- W3037-60
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Recombinant corresponding to aa46-90 and 228-298 connected by a gly-ser linker from mouse Wnt9b expressed in E. coli.
- Clone:
- 7H230
- Format:
- Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Mouse Wnt9b is a presumably secreted, glycosylated member of the Wnt family of developmental proteins. The 359aa mouse Wnt9b precursor contains a 336aa mature region with 24 conserved cysteines and one potential N-linked glycosylation site. Mature mouse Wnt9b shows 93% and 98% aa identity with mature human and rat Wnt9b, respectively, and 63% aa identity with Wnt9a. Wnt9b is expressed in late embryos and in adult kidney, with lesser amounts in brain and liver. Applications: Suitable for use in Western Blot and ELISA. Other applications not tested. Recommended Dilutions: Western Blot: 1-5ug/ml. KATOIII human gastric carcinoma cell line, T47D human breast cancer cell line, ME180 human cervical epithelial carcinoma cell line lysates. Optimal dilutions to be determined by the researcher. Western Blot Buffers: Wash Buffer: 25mM Tris, pH 7.4, 0.5M sodium chloride 0.05% Tween-20 Blocking Buffer: 5% nonfat dry milk in wash buffer Blotting Buffer: 2% nonfat dry milk in wash buffer Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice