W3037-10

Wnt8b (Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family Member)

Size

£761.00

SKU:: W3037-10
Purity:: Purified by Protein G affinity chromatography from hybridoma culture supernatant.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Rat
Immunogen:: Recombinant protein corresponding to aa22-52 and aa195-269, connected by a Gly-Ser linker from mouse Wnt8b, expressed in E. coli (AAD31816). Species Sequence Homology: rat 100%, canine 98% and human 97%.
Clone:: 7H100
Format:: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Extra Details:: Mouse Wnt-8b is a 40-50kD secreted glycoprotein member of the Wnt family of proteins. Mature mouse Wnt-8b is 329aa in length and contains 24 cysteines plus three potential N-linked glycosylation sites. Little is known about Wnt8b. It appears to play a role in forebrain development. Applications: Suitable for use in Immunohistochemistry and Direct ELISA. Other applications not tested. Recommended Dilutions: Immunohistochemistry: 8-25ug/ml detects Wnt-8b in immersion fixed frozen sections of embryonic mouse brain Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.