WNT3 (Proto-oncogene Wnt-3, Proto-oncogene Int-4 Homolog, INT4)

Size

£642.00

SKU:: W3035-55B
Purity:: Purified by immunoaffinity chromatography.
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Goat
Immunogen:: Synthetic peptide corresponding to CGRGHNTRTEKRKEK at the internal region of human WNT3. Species sequence homology: mouse, canine and rat.
Format:: Supplied as a liquid in Tris saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Extra Details:: Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Peptide ELISA: 1:16,000. Western Blot: 1-3ug/ml, ~40kD band observed in lysates of cell line HEK293 (calculated MW of 39.6kD according to NP_110380.1). Immunohistochemistry (Paraffin): 3-5ug/ml Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.