W3028-01
Wnt-5a (Protein Wnt-5a, Wnt5a, Wnt-5)
Size
£889.00
- SKU:
- W3028-01
- Purity:
- Purified by Protein G affinity chromatography from hybridoma culture supernatant.
- Storage Conditions:
- -20°C
- Supplier:
- United States Biological
- Host:
- Rat
- Immunogen:
- Recombinant protein corresponding to Gln38-Lys380 of mouse Wnt-5a expressed in CHO cells.
- Clone:
- 11C65
- Format:
- Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.
- Extra Details:
- Wnt-5a is a 49kD glycoprotein member of the Wnt family of developmental proteins. It is considered a class 3 Wnt based on its lack of transforming activity on C57MG cells. Genetic deletion of Wnt-5a interferes with developmental proximal-distal outgrowth of limbs and other structures. Wnt-5a can activate or inhibit b-catenin signaling depending on receptor context. Mature mouse Wnt-5a is a 342aa protein that contains 24 cysteines and four potential N-linked glycosylation sites. Mature mouse, rat and human Wnt-5a share 99% aa identity. Applications: Suitable for use in Immunohistochemistry, ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Immunohistochemistry (Frozen): 8-25ug/ml Immersion fixed frozen mouse embryo tissue sections. Western Blot: HeLa human cervical epithelial carcinoma cell line and mouse embryo (13 d.p.c) tissue. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
- Shipping Conditions:
- Blue Ice