W3005-75

Wnt Inhibiting Factor 1 (WIF-1)

Size

£576.00

SKU:: W3005-75
Purity:: Purified by Protein G affinity chromatography from hybridoma culture supernatant
Storage Conditions:: -20°C
Supplier:: United States Biological
Host:: Mouse
Immunogen:: Recombinant protein corresponding to Gly29-Trp379 from human WIF-1, expressed in NSO cells
Clone:: 8H102
Format:: Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with sterile PBS.
Extra Details:: WIF-1 is a secreted protein that binds Wnt proteins and antagonizes Wnt-induced signaling. WIF-1 expression is down regulated in several human cancers. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml Immunohistochemistry (Paraffin): 8-25ug/ml; Used to detect WIF-1 in immersion fixed paraffin-embedded human breast sections. Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.